ANANAS
http://www.whfoods.com/genpage.php?tname=foodspice&dbid=34
Ananas kuuluu myös silmälle edullisiin hedelmiin. Varsinkin tuoreena, jolloin siinä ei ole lisänä sokeriliemiä
kts. www.fineli.fi
Ananaksesta on löydetty maksaa suojaavia ominaisuuksia. esim parasetamolimyrkytystä vastustavaa ominaisuutta. Tässä allalolevassa on käytetty fermentoitua anansjuissia. . ( Netissä löytyy pannas vinegar thetynä kuorista fermentaatiolla. Tässä alla sanotaan etät juissista, Mutta onko taas juissi tehty koko hedelemistä, ei käy ilmi.
http://www.ncbi.nlm.nih.gov/pubmed/25699088
Chin Med. 2015 Feb 13;10:3. doi: 10.1186/s13020-015-0030-4. eCollection 2015.
Antioxidant effects of pineapple vinegar in reversing of paracetamol-induced liver damage in mice.
Abstract
BACKGROUND:
Pineapple (Ananas
comosus) was demonstrated to be hepatoprotective. This study aims to
investigate the reversing effects of pineapple vinegar on
paracetamol-induced liver damage in murine model.
METHODS:
Pineapple
juice was fermented via anaerobic and aerobic fermentation to produce
pineapple vinegar. Male BALB/c mice (n = 70) were separated into 7
treatment groups (n = 10). Pineapple vinegar (0.08 and 2 mL/kg BW) and
synthetic vinegar were used to treat paracetamol-induced liver damage in
mice. The hepatoprotective effects were determined by serum
biochemistry profiles (aspartate aminotransferase (AST), alanine
aminotransferase (ALT), alkaline phosphatase (ALP), and triglyceride
(TG)), liver antioxidant levels (ferric-reducing ability plasma (FRAP),
superoxide dismutase (SOD), malondialdehyde (MDA), nitric oxide (NO),
and reduced glutathione assays (GSH)) and histopathological examination
with hematoxylin and eosin (H&E) staining. The effects were further
evaluated by the expression levels of iNOS, NF-κB, and cytochrome P450
2E1 by quantitative real-time PCR and Western blot analyses. Vinegar
samples were also tested for in vitro antioxidant (FRAP,
2,2-diphenyl-2-picrylhydrazyl (DPPH), and total phenolic content (TPC)).
Soluble phenolic acid contents in the samples were identified by HPLC.
RESULTS:
Pineapple
vinegar contained 169.67 ± 0.05 μg GAE/mL of TPC, with
862.61 ± 4.38 μg/mL gallic acid as the main component. Oral
administration of pineapple vinegar at 2 mL/kg BW reduced serum enzyme
biomarker levels, including AST (P = 0.008), ALT (P = 0.006), ALP (P =
0.002), and TG (P = 0.006) after 7 days of paracetamol treatment.
Liver
antioxidant levels such as hepatic glutathione (P = 0.003), SOD
(P < 0.001), lipid peroxidation (P = 0.002) and FRAP (P <0 .001="" b="">were restored after the treatment.0>
Pineapple vinegar reduced the
expressions of iNOS (P = 0.003) and NF-kB (P = 0.003) and the
level of
NO (P = 0.003)
significantly. Pineapple vinegar also downregulated liver
cytochrome P450 protein expression.
CONCLUSIONS:
Oral
administration of pineapple vinegar at 0.08 and 2 mL/kg BW reduced
serum enzyme biomarker levels, restored liver antioxidant levels,
reduced inflammatory factor expressions, and down regulated liver
cytochrome P450 protein expression in paracetamol-induced liver damage
in mice.
MUITA LÄHTEITÄ
Luoga W, Mansur F, Buttle DJ, Duce IR, Garnett MC, Lowe A, Behnke JM.
J Helminthol. 2015 Mar;89(2):165-74. doi: 10.1017/S0022149X13000692. Epub 2013 Oct 31.
.
Romano B, Fasolino I, Pagano E, Capasso R, Pace S, De Rosa G, Milic N, Orlando P, Izzo AA, Borrelli F.
Mol Nutr Food Res. 2014 Mar;58(3):457-65. doi: 10.1002/mnfr.201300345. Epub 2013 Oct 1.
Dutta S, Bhattacharyya D.
J Ethnopharmacol. 2013 Nov 25;150(2):451-7. doi: 10.1016/j.jep.2013.08.024. Epub 2013 Sep 26.
The extract contains several proteins that were clustered under native condition. Proteomic studies indicated presence of fruit bromelain
as major protein constituent of the extract. It showed nonspecific
protease activity, gelatinolytic, collagenase, fibrinolytic, acid and
alkaline phosphatase, peroxidase, DNase and RNase activities along with
considerable anti-microbial property
Novaes LC, Ebinuma Vde C, Mazzola PG, Pessoa A Jr.
Biotechnol Appl Biochem. 2013 Sep-Oct;60(5):527-35. doi: 10.1002/bab.1121. Epub 2013 Sep 6.
Abstract
Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple (Ananas comosus
Merr.), and it is known for its clinical therapeutic applications, food
processing, and as a dietary supplement. The use of pineapple waste for
bromelain
extraction is interesting from both an environmental and a commercial
point of view, because the protease has relevant clinical potential. We
aimed to study the optimization of bromelain
extraction from pineapple waste, using the aqueous two-phase system
formed by polyethylene glycol (PEG) and poly(acrylic acid). In this
work, bromelain
partitioned preferentially to the top/PEG-rich phase and, in the best
condition, achieved a yield of 335.27% with a purification factor of
25.78. The statistical analysis showed that all variables analyzed were
significant to the process.